To complete the sequence of the AMV chayote isolate genome (named See-1), primers were designed from the contig sequences and used to amplify rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR) products spanning the 5′ and 3′ terminal regions of the three genomic RNAs using the SMARTer RACE cDNA Amplification Kit (Clontech, China). Mapping of reads on AMV genomic RNAs identified 4,209 AMV reads (1.26% of total reads) and allowed the scaffolding of the contigs into three scaffolds corresponding to the three AMV genomic RNAs. Contigs were then annotated by BLASTn and BLASTx comparison with the GenBank database, which allowed the identification of eight contigs of between 380 and 980 nucleotides (nt) sharing significant identity with alfalfa mosaic virus (AMV) genomic RNAs. Reads were assembled using CLC Genomics Workbench 11.1 (). Double-stranded RNA was extracted from symptomatic leaves of a single chayote plant and reverse transcribed, randomly amplified, and submitted to Illumina sequencing (Marais et al. Symptoms coalesced in some leaves, covering almost the whole foliar area. During spring 2019, some chayote plants showing bright yellow vein banding, rings, and lines were observed in a private garden in South Italy (Campania region). 698 ISSN: 0191-2917 Subject: Alfalfa mosaic virus, Bos, Chenopodium giganteum, Gynostemma pentaphyllum, Nicotiana benthamiana, Sechium edule, Solanum lycopersicum, double-stranded RNA, epidemiology, gardens, genes, genetic databases, genomics, nucleotides, reverse transcriptase polymerase chain reaction, sap, sequence analysis, spring, tobacco, Africa, Australia, Central America, China, Italy, New Zealand, Switzerland Abstract: Chayote (Sechium edule Sw.) is a vigorous perennial and climbing cucurbit, native to Mesoamerica, and cultivated for alimentary purposes in the American continent, Australia, New Zealand, South Europe, Asia, and Africa. Candresse Source: Plant disease 2021 v.105 no.3 pp. First Report of Alfalfa Mosaic Virus in Chayote in Italy Author: G.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |